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IACUC Protocol Form


Student Researchers - Frequently Asked Questions

1) How do I begin a research project using animals?

2) How do I add or remove people to/from an approved protocol?


3) My protocol expired - what should I do?

4) How do I change or add a procedure to an approved protocol?

5)  How can I get access to the animal facility?

6) Where should I put dirty cages?

7) Where can I find clean caging and supplies?

8) One of my animals looks sick - what should I do?

9) One of my animals needs to be euthanized, what is the proper procedure?

10) Where should I put the carcasses of animals?

11) What is the Raptor Center?

12) I was injured by an animal - what should I do?



  1) How do I begin a research project using animals?
    To begin research using animals, you must first have your protocol approved by the IACUC.  This protocol form is available here .  Once your protocol has been approved by the IACUC, you should find out whether animals need to be ordered by talking to the principal investigator or the facility supervisor.  If animals need to be ordered, this must be done by the PI through the animal facility supervisor using this form .  Animals from the facility cannot be used, nor can animals be ordered without an approved protocol.  

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2) How do I add or remove people to/from an approved protocol?
    To add or remove a person from an approved protocol, you must submit an addendum to your current protocol.  This addendum must be approved by the IACUC.  This form is available here .

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3) My protocol expired - what should I do?
    Protocols are approved for up to 3 years, after which time they expire.  You may submit an addendum with a reference to the previous approved protocol.

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4) How do I change or add a procedure to an approved protocol?
    To change a procedure, add a procedure, or increase the number of animals, you must submit an addendum to your current protocol, given that the changes fit criteria outlined by the IACUC.    

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5)  How can I get access to the animal facility?
    Facility access must be requested through the facility supervisor by the principal investigator.   Key access must be requested in the same manner.  

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6) Where should I put dirty cages?
    Dirty cages should be scraped clean and placed in the cage washing room - OlRi 165M for the facility staff.  Dirty bedding should be placed in the waste can that is enclosed by a filter hood in 165 M.  

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7) Where can I find clean caging and supplies?
    All clean caging, food and bedding may be found in room 165 N.  Be sure to consult the animal care manual for proper procedures for changing cages, and feeding and watering animals.  

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8) One of my animals looks sick - what should I do?
    Sick animals should be reported to the animal facility supervisor.  A health report form may be found on the door of the animal facility office -165 C.  This form should be completed according to the instructions found inside the folder.  

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9) One of my animals needs to be euthanized, what is the proper procedure?
    Rodents from ACS are usually euthanized using CO2, but all euthanization procedures should be included in your protocol.  A cylinder or CO2 is found in the corridor by the elevator.  Only those people who have been trained in euthanization procedures are allowed to euthanize animals.  If you are not trained, you may request training from the facility supervisor or your principal investigator.  If an animal needs immediate attention and you have not received adequate training, you should report the problem to the facility supervisor or your principal investigator.  See the AVMA Panel on Euthanasia for guidelines for non-rodent species

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10) Where should I put the carcasses of animals?
    Carcasses should be wrapped in a plastic bag found in the storage room 165N and placed in the freezer in the corridor by the elevator.  Animals fulfilling the following criteria may be donated to the Raptor Center and thus should be placed on the LEFT side of the freezer:  

1.  Eligible species include mice, rats, gerbils, hamsters, guinea pigs, chinchillas and rabbits of all sizes and ages.

2.  Eligible animals include: litter mates of transgenic or knock-out mice, retired breeders, 'surprise' litters, spontaneous deaths or illnesses, improperly ordered or surplus animals, normal control animals, bodies of those used for tissue harvest, sentinels, etc.
3.  Animals must be euthanized by cervical dislocation (small rodents) or carbon dioxide overdose. Animals that die spontaneously and are quickly refrigerated or frozen are also eligible. If an animal has received any anesthetic or euthanasia solution it is not eligible.
4.  Any animal that has received drugs or biohazard agents of any kind during the course of an experiment is not eligible. This includes anesthetics or antibiotics. This does not include viruses or retroviral vectors unless they are considered a zoonosis.
5.  Animals exhibiting signs of or dying from a spontaneous disease are also allowed as food. Examples of animals acceptable to the raptor center include those with ectoparasites (fur mites), abscesses, fractures, or tumors. It is not acceptable to leave a suffering animal alive until the designated pick-up day. These animals should be euthanized and the body refrigerated or frozen.
6.  The Raptor Center can also use animals that have been properly refrigerated or frozen. When refrigerating or freezing mice or other small rodents, place them neatly in a plastic bag no more than three animals deep. Rats, guinea pigs and rabbits should be refrigerated or frozen singly.


Animals that are not suitable for donation should be placed on the RIGHT side of the freezer to be incinerated.  

Any death that is sudden or suspicious should be immediately reported to the facility supervisor for a necropsy.  Inform the supervisor of the death and place the carcass on the RIGHT side of the freezer.  Mark the bag with the date of death and write "necropsy" on the bag for the supervisor.  


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11) What is the Raptor Center?
   
    The Raptor Center is a center for the rehabilitation of birds of prey found at the University of Minnesota College of Veterinary Medicine.  Rodents fulfilling the above criteria are donated to the Center as food.  
More information on the Center may be found on their homepage.

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12) I was injured by an animal - what should I do?
    A first aid kit may be found in the locker room 165 A.  This kit contains bandages and topical antibacterial ointments.  Antibacterial soap can be found in the cage washer room 165 M for cleansing wounds.  All bites and scratches that break the skin should be thoroughly washed and bandaged.  Monitor injuries for signs of infection or allergy.  Any serious injuries should be reported to the facility supervisor or your principal investigator.  

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Last updated: 01/09/2003
Send comments and questions to: mensink@macalester.edu



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