Fluorescence Microscopy

What is fluorescence?
Wavelength Spectra
Excitation Spectra
Stokes' Shift
Advantages of Fluorescence Microscopy
Light Sources
Filters
The Path of Light
Staining Specific Cell Structures
Twelve Cell DAPI Stained Embryo
Related Links
References


What is fluorescence?

     Fluorescence is the property of some atoms or molecules to  absorb light of a particular wavelength and re-emit later at a longer wavelength.  The interval between absorption and re-emission is termed the fluorescence  lifetime.

Wave length Spectra
Excitation Spectra

Stokes' Shift

Advantages of Fluorescence Microscopy

  Fluorescent microscopy has many advantages over normal microscopy, including:
Light Sources
Filters
The Path of Light

   Light follows a specific path through the microscope.
Specific Cell Structures

   A great many specific cell structures can be stained and viewed with a fluorescence microscope. These cell structures include Microtubules, Actin, DNA, Mitochondria, Golgi and ER.

Twelve Cell DAPI Stained Embryo
   This image is a twelve cell C. elegans embryo stained with DNA-binding DAPI. The picture was taken under 63x magnification with a fluorescence microscope.




Related Links:


http://www.microscopy.fsu.edu/primer/java/lightpaths/bx51fluorescence/bx51.html


http://www.itg.uiuc.edu/publications/techreports/99-006/


http://www.espn.go.com

References


Herman, Brian. Fluorescence Microscopy. Springer Pub.; New York, 1998.

The above links were also quite handy.





This page was written and compiled by Ben Byrd.