Morpholino Technology

DNA

Macalester College
December 2002
Garett M Padilla

Background
Synthesis
Properties of Morpholinos
Applications of Morpholinos

Methods of Delivery
Controls
Alternatives to Morpholinos
Links


Background

Synthesis

   Click on the link below to see subunit synthesis :
       
       Morpholinos function by binding to the 5' UTR region and prevent the ribosomal initiation complex  from binding to the cognate mRNA.  Morpholinos can also enter the nucleus and base pair with a "pre-mRNA"
before it has been post-transcriptionally modified.
 

Properties of Morpholinos


Applications of Morpholinos

Methods of Delivery

Controls
Alternatives to Morpholinos



  

Links

Morpholino Synthesis

Gene Tools Inc.

Antisense Technologies Comparison 


Leading References

Hudziak, Barofsky, Barofsky, Weller, Huang, Weller (1996).  Resistance of Morpholino phophorodiamidate oligomers to enzymatic degradation. Antisense Nucleic Acid Drug Development.  6,  267.

Partridge, Vincent, Matthews, Puma, Stein, Summerton (1996).  A simple method for delivering Morpholino antisense oligos into the cytoplasm of cells. Antisense Nucleic Acid Drug Development.  6,  169.

Stein (1995). Does antisense exist?  Nature Medicine 1. 1119.