CURRICULUM VITAE

MARY K. MONTGOMERY

Macalester College

Biology Department

1600 Grand Avenue

St. Paul, MN 55105

(651) 696-6425 (office)

(651) 696-8174 (lab)

email: montgomery@macalester.edu

website: http://www.macalester.edu/~montgomery/

I. EDUCATION

Post-Doc Developmental Biology 1994-1998

Carnegie Institution of Washington, Department of Embryology

Advisor: Dr. Andrew Fire

Ph.D. Biological Sciences 1993

University of Southern California, Los Angeles, CA

Dissertation:                         Development of the symbiotic light organ of Euprymna scolopes Berry (Cephalopoda: Sepiolidae)

Advisor: Dr. Margaret McFall-Ngai

B.A. Biology (with honors) 1986

Immaculata College, PA

II. ACADEMIC EMPLOYMENT

Full-Time Appointments

1998- present Macalester College, Biology Department, Assistant Professor

Part-Time Visiting Appointment

Fall 1997 Johns Hopkins University, Dept. of Biological Sciences, Instructor

III. PUBLICATIONS

Peer-Reviewed Research Articles

1. Montgomery, MK, S Xu, A Fire (1998) RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans. Proc Natl Acad Sci 95: 15502-15507.
2. Montgomery, MK and M McFall-Ngai (1998) Late postembryonic development of the symbiotic light organ of Euprymna scolopes. Biological Bulletin (MBL, Woods Hole, MA) 195: 326-36.
3. Fire, A, S Xu, MK Montgomery, S Kostas, S Driver and C Mello (1998) Potent and specific genetic interference mediated by double-stranded RNA in Caenorhabditis elegans . Nature 391: 806-811.
4. Kelly, WG, S Xu, MK Montgomery and A Fire (1997) Distinct requirements for somatic and germline expression of a generally expressed Caenorhabditis elegans gene. Genetics 146: 227-238.
5. Mickey, KM, CC Mello, MK Montgomery, A Fire and JR Priess (1996) An inductive interaction in 4-cell stage C. elegans embryos involves APX-1 expression in the signalling cell. Development 122(6): 1791-1798.
6. Montgomery, MK and P Kremer (1995) Transmission of symbiotic dinoflagellates through the sexual cycle of the host scyphozoan Linuche unguiculata . Marine Biology 124: 147-155.
7. Montgomery, MK and M McFall-Ngai (1994) Bacterial symbionts induce host organ morphogenesis during early postembryonic development of the squid Euprymna scolopes. Development 120: 1719-1729.
8. Montgomery, MK and M McFall-Ngai (1993) Embryonic development of the light organ of the sepiolid squid Euprymna scolopes Berry. Biological Bulletin (MBL, Woods Hole, MA) 184: 296-308.
9. Weis, VM, MK Montgomery and M McFall-Ngai (1993) Enhanced production of an ALDH-like protein in the bacterial light organ of the sepiolid squid Euprymna scolopes . Biological Bulletin (MBL, Woods Hole, MA) 184: 309-321.
10. Montgomery, MK and M McFall-Ngai (1992) The muscle-derived lens of a squid bioluminescent organ is biochemically convergent with the ocular lens. Evidence for recruitment of aldehyde dehydrogenase as a predominant structural protein. Journal of Biological Chemistry 267: 20999-21003.
11. McFall-Ngai, M and MK Montgomery (1990) The anatomy and morphology of the adult bacterial light organ of Euprymna scolopes Berry (Cephalopoda: Sepiolidae). Biological Bulletin (MBL, Woods Hole, MA) 179: 332-339.

Book Chapters and Review Articles

1. Montgomery, MK (in press) RNAi: Historical overview and significance. Book chapter for RNA interference, editing, and modification (J. Gott, Ed) in Methods in Molecular Biology series, Humana Press: New York.
2. Montgomery, MK (in press) The use of double-stranded RNA to knock down specific gene activity. Book chapter for Mobile Genetic Elements: Protocols and Genomic Applications (W. Miller, Ed.) in Methods in Molecular Biology series, Humana Press: New York.
3. Norman, J and MK Montgomery (2001) RNAi and cosuppression: double-stranded RNA as an agent of sequence-specific genetic silencing in animal and plants in Molecular Biology of Double-stranded RNA: Concepts and Applications in Agriculture, Forestry, and Medicine , S. M. Tavantzis, Ed. (pp. 1-36) CRC Press: New York.
4. Montgomery, MK and A Fire (1998) Double-stranded RNA as a mediator in sequence-specific gene silencing and co-suppression. Trends in Genetics 14: 255-258.
5. Montgomery, MK and M McFall-Ngai (1994) The inductive role of bacterial symbionts in the morphogenesis of a squid light organ. American Zoologist 35: 372-380.

Non-Reviewed Scholarly Contributions

• “Cell Evolution” in Biology for Students , an encyclopedia to be published by Macmillan Reference USA
• Website on RNAi: http://www.macalester.edu/~montgomery/RNAi.html (used to supplement Scott Gilbert’s Developmental Biology  textbook at book’s companion website: see http://www.devbio.com/chap04/link0408a.shtml)

IV. PRESENTATIONS (^abstract published, undergraduate co-author, primary presenter )

Presentations at National or International Conferences

Talks

1. V Descotte and MK Montgomery “Differing susceptibilities to systemic RNAi within the Caenorhabditis clade” 14^th International C. elegans Meeting (Los Angeles, CA) Jul 2003
2. J Norman, E Bishop, and MK Montgomery "Potential for cross-interference with RNAi" 13^th International C. elegans Meeting (Los Angeles, CA) Jun 200
3. MK Montgomery and A Fire “Regulation of maternal mRNA activity” Eleventh International C. elegans Meeting (Madison, WI) Jun 1997
4. Invited speaker for symposium entitled “The Role of Cell-Cell Interactions and Environmental Stimuli in the Development of Marine Invertebrates and Animals” Annual Meeting of the American Society of Zoologists (currently Society for Integrative and Comparative Biology) (Los Angeles, CA) 1993
5. ^MK Montgomery and M McFall-Ngai “Embryological development of a squid symbiotic light organ” Annual Meeting of American Society of Zoologists (Atlanta, GA) (American Zoologist 32: 80A) 1992
6. ^MK Montgomery and M McFall-Ngai “The influence of bacterial symbionts on the early morphogenesis of the light organ of the sepiolid squid, Euprymna scolopes ” Annual Meeting of the American Society of Zoologists (Vancouver, BC) (American Zoologist 31:14A) 1991
7. ^MK Montgomery and P Kremer “Direct transmission of algal symbionts through the sexual cycle of the host scyphozoan, Linuche unguiculata ” Annual Meeting of the American Society of Zoologists (Boston, GA) (American Zoologist 29: 146A) 1989

Posters

1. ^V Descotte and MK Montgomery “Differing susceptibilities to systemic RNAi within the Caenorhabditis clade” 62^nd Annual Meeting of the Society for Developmental Biology (Boston, MA) Aug 2003
   
2. ^MK Montgomery “Collaborative research with undergraduate students: an assembly line model” 61 ^st Annual Meeting of the Society for Developmental Biology (Madison, WI) (Dev Biol 247:441) Jul 2002
3. MK Montgomery “Differing susceptibilities to systemic RNAi within the Caenorhabditis clade” European C. elegans Meeting (Paestum, Italy) May 2002 Also presented at the 13 ^th International C. elegans Meeting (Los Angeles, CA) Jun 2001
4. ^ J Norman, E Bishop, and MK Montgomery "Potential for cross-interference with RNAi"
   60th Annual Meeting of the Society for Developmental Biology (Seattle, WA) (Dev Biol 235: 234) Jul 2001
5. ^ G. Padilla , S. Goetz , MK Montgomery "Tools for nematode evodevo studies"
   60th Annual Meeting of the Society for Developmental Biology (Seattle, WA) (Dev Biol 235: 234) Jul 2001
6. ^MK Montgomery “Breaking out of the model paradigm: using the comparative approach in undergraduate investigative labs” 59 ^th Annual Meeting of the Society for Developmental Biology (Boulder, CO) (Dev Biol 222: 234) Jun 2000
7. ^J Norman , E Bishop , and MK Montgomery “Using RNAi to study SKN-1 and other homologs governing early nematode development” 59^th Annual Meeting of the Society for Developmental Biology (Boulder, CO) (Dev Biol 222: 237) Jun 2000
8. ^MK Montgomery “Lecture/lab combo: P-granule immunostaining in C. elegans ” 58^th Annual Meeting of Society for Developmental Biology (Charlottesville, VA) Jun 1999
9. ^K Galey, J Norman and MK Montgomery “The ‘minimal dsRNA unit’ needed for RNAi in nematodes” 58^th Annual Meeting of Society for Developmental Biology (Charlottesville, VA) Jun 1999
10. J Norman , K Galey, and MK Montgomery “RNAi: how low can you go?”
    Twelfth International C. elegans Meeting (Madison, WI) Jun 1999

Workshops

• Co-organizer and co-presenter (with Eric Cole, St. Olaf College), Education Workshop II, “Laboratories for developmental biology—two different approaches" 61^st Annual Meeting of the Society for Developmental Biology (Madison, WI) Jul 2002
• Organizer and presenter, RNAi Workshop
  58^th Annual Meeting of Society for Developmental Biology (Charlottesville, VA) Jun 1999
• Co-organizer (with Tom Evans, UW Madison) RNA injection techniques Workshop
  Tenth International C. elegans Meeting (Madison, WI) Jun 1995

Presentations at Regional Conferences

Talks

1. MK Montgomery and A Fire “RNAi: the mystery continues to unfold” 1998 MidWest C. elegans Meeting (Chicago, IL) Jun 1998
2. Invited speaker for symposium entitled “Influences of Bacteria on Development in Higher Plants and Animals” West Coast Developmental Biologists Meeting Mar 1993

Posters

1. J Norman, E Bishop , and MK Montgomery “Using RNAi to study SKN-1 and other homologs governing early nematode development” 2000 MidWest C. elegans Meeting (Minneapolis, MN) Jun 2000
2. MK Montgomery , K Galey , and J Norman “Using RNAi to study maternal gene function in ‘non-model’ nematodes” The Developmental Basis of Evolutionary Change (U of Chicago) May 1999
3. MK Montgomery and A Fire “Analysis of posttranscriptional regulation in the early embryo” East Coast C. elegans Meeting (Rutgers U., NJ) Jun 1996

Workshops

• Organizer and co-presenter (with Eric Cole, St. Olaf College), Education Workshop 41st Annual Midwest Developmental Biology Meeting (Columbia, MO) May 2001

Invited Lectures

1. “Repression of gene activity using double-stranded RNA (RNAi): Evolutionary conservation and diversification in mechanism and function” University of Koln, Germany Apr 2002
2. “Repression of gene activity using double-stranded RNA (RNAi): Evolutionary conservation and diversification in mechanism and function” University of Dusseldorf, Germany May 2002
3. “Stop making sense: RNAi and gene silencing” St. Olaf College, MN Mar 2002
4. “RNAi: silencing genes both naughty and nice” Carleton College, MN Oct 2001
5. “RNAi: faster, cheaper, better?” Oregon State University, OR Feb 2001
6. “Use of transgene reporters to study gene expression” C. elegans course, Cold Spring Harbor Laboratories, NY Nov 1999
7. University of Colorado Medical School, Denver, CO (invited by graduate students in the Molecular Biology Program for annual symposium, “Reversing into the Future: Functional Genomics and RNA Interference in Flies and Worms”) May 1999

V. AWARDS, RECOGNITION, AND GRANTS

External Grants

• 2001-2003 National Science Foundation, Major Research Instrumentation Grant “Acquisition of Cell Imaging and Cell Laser Ablation Systems for Developmental Biology Research” ($115,000) (+ $8000 matching funds)
• 1999-2001 National Science Foundation, Course, Curriculum, Laboratory Improvement Grant “Integration of a Developmental Biology Program into a Research-Based Curriculum” ($35,000) (+ Macalester matching funds)
  
  

Internal Support

• 1999-2003 Hughes Student/Faculty Collaborative Research Program Award (14 students supported for summer research)
• 2002 Wallace Faculty Travel grant
• 2001 “Creation of online quizzes to support introductory biology courses” Mellon Technology Grant

Support for Post-Doctoral and Graduate Work

• 1995-1997    National Research Service Award (NIH Training Grant Post-doctoral Fellowship),
• 1990-1993    Achievement Reward for College Scientists (ARCS Foundation, L.A. Chapter)
• 1986-1991    Dean's Fellowship (University of Southern California)
• 1990-1991    Biomedical Research Support Grant (NIH)
• 1990              Sigma Xi, Grants-in-Aid-of-Research
• 1988-1989    Biomedical Research Support Grant (NIH), academic year of 1988-89

Undergraduate Awards and Scholarships

• 1982-1986 Presidential Scholarship (Immaculata College, PA)
• 1986 Recipient of the Adeline E. Donio Medal for Biology, Immaculata College

VI. TEACHING

1998-present Macalester College, St. Paul, MN.

Courses Taught

First Year Seminars

The Unity and Diversity of Life

Genetics

Introductory Courses

Cell Biology

Genetics

Intermediate Courses

Developmental Biology

Advanced Courses

Research in Developmental Biology/Molecular Genetics

Seminar in Molecular Evolution

Fall 1997 Johns Hopkins University

Undergraduate Seminar: The Evolution of Developmental Mechanisms

Honors Research Advisor

Research performed in my laboratory:

2003    Veronica Descotte    Thesis:  Characterization of the RNA interference response within the Caenorhabditis clade
       (Graduate program in Molecular, Cellular, Developmental Biology, and Genetics, University of Minnesota)
2001    Sarah Goetz    Thesis: Evolution of the developmental determinant skn-1 in nematodes
       (Graduate program in Molecular, Cell, and Developmental Biology, University of North Carolina, Chapel Hill)
2000    Jeff Norman    Thesis: Utilizing the reverse genetic technique RNA interference to study homologous gene function in the nematodes C. elegans and C. briggsae
       (MD program, University of Minnesota)

Research performed at another institution:

2002 Whitney Smalley Thesis: The role of EP₂ in pregnancy in mice

Summer Research Advisor

Summer 1999 Jeff Norman (’00)

Erin Bishop (’01)

Adam Hankins (’01)

Chinelo Njaka (’01)

Emi Baldoni (’02)

Summer 2000 Erin Bishop (’01)

Sarah Goetz (’01)

Jeremy Jensen (’02)

Garett Padilla (’03)

Summer 2001 Garett Padilla (’03)

Osamudiame Uzzi (’04)

Summer 2003 Meghan Cagley (’04)

Holly Swanson (’04)

Rachael Harlos (’06)

VII. SERVICE

Membership in Professional Societies

Genetics Society of America

Society for Developmental Biology

Council on Undergraduate Research

Professional Service

Ad hoc reviewer for the following:

National Science Foundation

National Institutes of Health

USDA

Murdock College Research Program for Life Sciences

I have reviewed articles for the following journals:

Developmental Biology

Genetics

Marine Biology

Journal of the Marine Biological Association of the United Kingdom

Community Service

Judge for Ramsey Junior High Science Fair Nov 2002

Judge for undergraduate oral presentations at Annual Meeting of

the Minnesota Academy of Science 1999

Macalester College Service

Educational Policy and Governance Committee elected Spring 2003

Health Professions Advisory Committee 2002-present

Jardetzky Lectureship Committee 2001-present

Lilly Grant Symposium Committee 2003

Public Scholarship for Social Change Working Group 2003

BioMath Curriculum Committee 2001-02

Representative for the ACM Committee on Women’s Concerns 2001

Biology Department Search Committee for position in Plant Biology 2000

Biology Department Search Committee for position in Biochemistry 1999

Biology Search Committee for position in Animal Physiology 1999

Biology Search Committee for Ordway Naturalist 1999

Women and Genders Studies Steering Committee 1999-2001

Hughes Science Summer Institute 1999, 2000, 2001, 2003

(contributed to program by being a research mentor, academic advisor, and participant in several workshops)